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rat igg 2a isotype control antibody  (R&D Systems)


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    Structured Review

    R&D Systems rat igg 2a isotype control antibody
    Rat Igg 2a Isotype Control Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 39 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+igg+2a+isotype+control/Mouse+CXCR2%2FIL-8RB+Antibody/pmc11949021-210-25-34
    Average 93 stars, based on 39 article reviews
    rat igg 2a isotype control antibody - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: PhosY-secretome profiling combined with kinase-substrate interaction screening defines active c-Src-driven extracellular signaling
    Article Snippet: .. Anti-c-Src antibodies used for blocking experiments: rabbit anti-Src mAb1 (32G6, biotinylated) (Cell Signaling, #8077), rabbit (DA1E mAb IgG XP Isotype control, biotinylated) (Cell Signaling, #4096), mouse anti-Src mAb2 (Clone 327) (Abcam, #ab16885), rabbit IgG (Abcam, #ab172730), mouse anti-Src mAb3 (L4A1) (Cell Signaling, #2110), rabbit anti-Src mAb4 (36D10) (Cell Signaling, #2109), rabbit anti-Src mAb5 (32G6) (Cell Signaling, #2123), mouse anti-Src mAb6 (clone 327537) (R&D systems, MAB3389), mouse IgG 2A Isotype control (Clone #20102) (R&D systems, MAB003), rabbit pAb (aa 84–110) (custom antibody) (see ). ..

    Control:

    Article Title: PhosY-secretome profiling combined with kinase-substrate interaction screening defines active c-Src-driven extracellular signaling
    Article Snippet: .. Anti-c-Src antibodies used for blocking experiments: rabbit anti-Src mAb1 (32G6, biotinylated) (Cell Signaling, #8077), rabbit (DA1E mAb IgG XP Isotype control, biotinylated) (Cell Signaling, #4096), mouse anti-Src mAb2 (Clone 327) (Abcam, #ab16885), rabbit IgG (Abcam, #ab172730), mouse anti-Src mAb3 (L4A1) (Cell Signaling, #2110), rabbit anti-Src mAb4 (36D10) (Cell Signaling, #2109), rabbit anti-Src mAb5 (32G6) (Cell Signaling, #2123), mouse anti-Src mAb6 (clone 327537) (R&D systems, MAB3389), mouse IgG 2A Isotype control (Clone #20102) (R&D systems, MAB003), rabbit pAb (aa 84–110) (custom antibody) (see ). ..

    Article Title: Application of small molecule FPR1 antagonists in the treatment of cancers
    Article Snippet: .. Two Eppendorfs containing 30–50 × 10 4 cells each were treated with 20 μL of anti-hFPR1 (PE-conjugated, R&D system, FAB3744P) and mouse IgG 2A isotype control (PE-conjugated, R&D system, IC003G). .. Both tubes were incubated in the dark at 0 °C for 45 min. After washing with 1% BSA in PBS, the cells were analysed using a BD Accuri C6 plus flow cytometer.

    Article Title: EphA4 may contribute to microvessel remodeling in the hippocampal CA1 and CA3 areas in a mouse model of temporal lobe epilepsy
    Article Snippet: A total of 3 days following pilocarpine treatment, 12 of the treated mice were divided into 4 groups (n=3 per group), which subsequently received continuous intrahippocampal infusion in the left side using ALZET ® 1007D micro-osmotic pumps (DURECT Corporation, Cupertino, CA, USA) with standard ALZET brain infusion kits. .. The contents of the pumps were as follows: Artificial cerebrospinal fluid (ACSF) negative control group, in which the pump contained only ACSF (pH 7.42) containing 124 mM NaCl, 3 mM KCl, 2.4 mM CaCl 2 , 2.4 mM MgSO 4 , 1.25 mM KH 2 PO 4 , 26 mM NaHCO 3 and 10 mM D-glucose; IgG 2A control group, using a biologically non-active IgG in ACSF, in which the pump contained 50 μg/ml mouse IgG 2A isotype control (catalog no. MAB003; R&D Systems, Inc.) in ACSF; non-clustered (NC)-ephrin-A5-Fc group, in which the pump contained 50 μg/ml unclustered ephrin-A5-Fc (catalog no. 7396-EA-050; R&D Systems, Inc.) in ACSF; and clustered (C)-ephrin-A5-Fc group, in which the pump contained pre-clustered ephrin-A5-Fc (50 μg/ml ephrin-A5-Fc and 500 μg/ml goat anti-mouse IgG-Fc, catalog no. G-202-C; R&D Systems, Inc.) in ACSF. ..

    Article Title: Cholesterol depletion decreases adhesion of non-small cell lung cancer cells to E-selectin
    Article Snippet: .. Mouse anti-human CD44 antibody (clone 2C5; Cat. No. BBA10), mouse IgG 2A isotype control (Cat. No. MAB0031), and anti-mouse IgG fluorescein-conjugated antibody (Cat. No. F0103B) were purchased from R&D Systems (Minneapolis, MN). .. Mouse anti-human sLe A antibody (clone 116-NS-19-9; Cat. No. MA5-12421) and PE-conjugated mouse IgG 1 isotype control (Cat. No. 12-4714-42) were purchased from Invitrogen (Waltham, MA).

    Immunostaining:

    Article Title: Recognition of Bacterial Signal Peptides by Mammalian Formyl Peptide Receptors
    Article Snippet: For cell surface expression studies, cells were fixed for 4 min in methanol-free paraformaldehyde (Polysciences Inc.), 4% in PBS. .. For immunostaining, cells were blocked with 5% heat-inactivated FCS in PBS for 30 min and incubated overnight with the following monoclonal primary antibodies: anti-human FPR1 mouse IgG 2A (R&D Systems, MAB3744, 1 μg/ml); mouse IgG 2A isotype control (R&D Systems, MAB0031, 1 μg/ml); anti-human CD14 mouse IgG1,κ (Biolegend, 325601, 0.5 μg/ml); mouse IgG1,κ isotype control (Biolegend, 400101, 0.5 μg/ml). .. Staining was obtained by 45-min incubation with a polyclonal donkey anti-mouse Alexa Fluor 555 antibody (Invitrogen, 2 μg/ml) and a counterstaining of cell nuclei (Hoechst, 33342, 1 μg/ml).

    Incubation:

    Article Title: Recognition of Bacterial Signal Peptides by Mammalian Formyl Peptide Receptors
    Article Snippet: For cell surface expression studies, cells were fixed for 4 min in methanol-free paraformaldehyde (Polysciences Inc.), 4% in PBS. .. For immunostaining, cells were blocked with 5% heat-inactivated FCS in PBS for 30 min and incubated overnight with the following monoclonal primary antibodies: anti-human FPR1 mouse IgG 2A (R&D Systems, MAB3744, 1 μg/ml); mouse IgG 2A isotype control (R&D Systems, MAB0031, 1 μg/ml); anti-human CD14 mouse IgG1,κ (Biolegend, 325601, 0.5 μg/ml); mouse IgG1,κ isotype control (Biolegend, 400101, 0.5 μg/ml). .. Staining was obtained by 45-min incubation with a polyclonal donkey anti-mouse Alexa Fluor 555 antibody (Invitrogen, 2 μg/ml) and a counterstaining of cell nuclei (Hoechst, 33342, 1 μg/ml).

    Negative Control:

    Article Title: EphA4 may contribute to microvessel remodeling in the hippocampal CA1 and CA3 areas in a mouse model of temporal lobe epilepsy
    Article Snippet: A total of 3 days following pilocarpine treatment, 12 of the treated mice were divided into 4 groups (n=3 per group), which subsequently received continuous intrahippocampal infusion in the left side using ALZET ® 1007D micro-osmotic pumps (DURECT Corporation, Cupertino, CA, USA) with standard ALZET brain infusion kits. .. The contents of the pumps were as follows: Artificial cerebrospinal fluid (ACSF) negative control group, in which the pump contained only ACSF (pH 7.42) containing 124 mM NaCl, 3 mM KCl, 2.4 mM CaCl 2 , 2.4 mM MgSO 4 , 1.25 mM KH 2 PO 4 , 26 mM NaHCO 3 and 10 mM D-glucose; IgG 2A control group, using a biologically non-active IgG in ACSF, in which the pump contained 50 μg/ml mouse IgG 2A isotype control (catalog no. MAB003; R&D Systems, Inc.) in ACSF; non-clustered (NC)-ephrin-A5-Fc group, in which the pump contained 50 μg/ml unclustered ephrin-A5-Fc (catalog no. 7396-EA-050; R&D Systems, Inc.) in ACSF; and clustered (C)-ephrin-A5-Fc group, in which the pump contained pre-clustered ephrin-A5-Fc (50 μg/ml ephrin-A5-Fc and 500 μg/ml goat anti-mouse IgG-Fc, catalog no. G-202-C; R&D Systems, Inc.) in ACSF. ..



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